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Typical Testing Data/Standard Curve (for reference only)

Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) RTU ELISA Kit | NDUFS1 rtu elisa kit

Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit

Gene Names
NDUFS1; CI-75k; CI-75Kd; PRO1304
Reactivity
Human
Synonyms
NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1); Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit; CI-75Kd; NADH-Coenzyme Q Reductase; NADH Dehydrogenase Iron-Sulfur Protein 1; NADH-ubiquinone oxidoreductase 75 kDa subunit; mitochondrial; NDUFS1 rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
691
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.105ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NDUFS1 rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NDUFS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NDUFS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NDUFS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NDUFS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75,375 Da
NCBI Official Full Name
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform 2
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase core subunit S1
NCBI Official Symbol
NDUFS1
NCBI Official Synonym Symbols
CI-75k; CI-75Kd; PRO1304
NCBI Protein Information
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
UniProt Protein Name
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
UniProt Gene Name
NDUFS1
UniProt Synonym Gene Names
CI-75kD

NCBI Description

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

Research Articles on NDUFS1

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Product Notes

The Human NDUFS1 ndufs1 (Catalog #AAA4501313) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4501313 RTU ELISA Kit recognizes Human NDUFS1. It is sometimes possible for the material contained within the vial of "NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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