Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit | NDUFS1 elisa kit

Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit

Cat. Num. AAA455832

• Gene Names: NDUFS1; CI-75k; CI-75Kd; PRO1304
• Reactivity: Human
• Synonyms: NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1); Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit; NDUFS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NDUFS1. No significant cross-reactivity or interference between NDUFS1 and analogues was observed.
• Sequence Length: 691

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.105ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NDUFS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NDUFS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NDUFS1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NDUFS1 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NDUFS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NDUFS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NDUFS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NDUFS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 316983154
• NCBI GeneID: 4719
• NCBI Accession #: NP_001186910.1
• NCBI GenBank Nucleotide #: NM_001199981.1
• UniProt Accession #: P28331; Q53TR8; Q8N1C4; Q8TCC9; B4DIN9; B4DJA0; B4DPG1; B4DUC1; E7ENF3
• Molecular Weight: 75,375 Da
• NCBI Official Full Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform 2
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase core subunit S1
• NCBI Official Symbol: NDUFS1
• NCBI Official Synonym Symbols: CI-75k; CI-75Kd; PRO1304
• NCBI Protein Information: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• UniProt Protein Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• UniProt Gene Name: NDUFS1
• UniProt Synonym Gene Names: CI-75kD

NCBI Description

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.