Human NDUFS1 (NADH-ubiquinone oxidoreductase core subunit S1) ELISA Kit | NDUFS1 elisa kit

Human NDUFS1 (NADH-ubiquinone oxidoreductase core subunit S1) ELISA Kit

Cat. Num. AAA7608118

• Gene Names: NDUFS1; CI-75k; CI-75Kd; PRO1304
• Reactivity: Human
• Synonyms: NDUFS1 (NADH-ubiquinone oxidoreductase core subunit S1); Human NDUFS1 (NADH-ubiquinone oxidoreductase core subunit S1) ELISA Kit; NADH-ubiquinone oxidoreductase 75 kDa subunit; mitochondrial; Complex I-75kD; CI-75kD; NDUFS1; NDUFS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NDUFS1. No significant cross-reactivity or interference between NDUFS1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NDUFS1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 316983154
• NCBI GeneID: 4719
• NCBI Accession #: NP_001186910.1
• NCBI GenBank Nucleotide #: NM_001199981.1
• UniProt Accession #: P28331; Q53TR8; Q8N1C4; Q8TCC9; B4DIN9; B4DJA0; B4DPG1; B4DUC1; E7ENF3
• Molecular Weight: 79 kD
• NCBI Official Full Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial isoform 2
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
• NCBI Official Symbol: NDUFS1
• NCBI Official Synonym Symbols: CI-75k; CI-75Kd; PRO1304
• NCBI Protein Information: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; complex I 75kDa subunit; complex I, mitochondrial respiratory chain, 75-kD subunit; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
• UniProt Protein Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• UniProt Gene Name: NDUFS1
• UniProt Synonym Gene Names: CI-75kD
• UniProt Entry Name: NDUS1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

NDUFS1: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I 75 kDa subunit family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; Mitochondrial; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial respiratory chain complex I

Molecular Function: 2 iron, 2 sulfur cluster binding; protein binding; electron carrier activity; NADH dehydrogenase (ubiquinone) activity; 4 iron, 4 sulfur cluster binding; metal ion binding

Biological Process: ATP metabolic process; cellular metabolic process; apoptotic mitochondrial changes; mitochondrial electron transport, NADH to ubiquinone; regulation of mitochondrial membrane potential; cellular respiration

Disease: Mitochondrial Complex I Deficiency