Human Na-K-Cl Cotransporter 2 (NKCC2) RTU ELISA Kit | NKCC2 rtu elisa kit

Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit

Cat. Num. AAA4501384

• Gene Names: SLC12A1; BSC1; NKCC2
• Reactivity: Human
• Synonyms: Na-K-Cl Cotransporter 2 (NKCC2); Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit; SLC12A1; NKCC2; Solute Carrier Family 12 Member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; NKCC2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 1099

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 13.8pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NKCC2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NKCC2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NKCC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NKCC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NKCC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 134254459
• NCBI GeneID: 6557
• NCBI Accession #: NP_000329.2
• NCBI GenBank Nucleotide #: NM_000338.2
• UniProt Accession #: Q13621; A8JYA2; E9PDW4
• Molecular Weight: – Da
• NCBI Official Full Name: solute carrier family 12 member 1 isoform A
• NCBI Official Synonym Full Names: solute carrier family 12 member 1
• NCBI Official Symbol: SLC12A1
• NCBI Official Synonym Symbols: BSC1; NKCC2
• NCBI Protein Information: solute carrier family 12 member 1
• UniProt Protein Name: Solute carrier family 12 member 1
• UniProt Gene Name: SLC12A1
• UniProt Synonym Gene Names: NKCC2

NCBI Description

This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]

Uniprot Description

Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.