Human Sodium/Glucose Cotransporter 1 (SGLT1) RTU ELISA Kit | SGLT1 rtu elisa kit

Human Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit

Cat. Num. AAA4502469

• Gene Names: SLC5A1; NAGT; SGLT1; D22S675
• Reactivity: Human
• Synonyms: Sodium/Glucose Cotransporter 1 (SGLT1); Human Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit; SLC5A1; NAGT; Solute Carrier Family 5 Member 1; High affinity sodium-glucose cotransporter; Na(+)/glucose cotransporter 1; SGLT1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 664

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SGLT1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SGLT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SGLT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SGLT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SGLT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507031
• NCBI GeneID: 6523
• NCBI Accession #: NP_000334.1
• NCBI GenBank Nucleotide #: NM_000343.3
• UniProt Accession #: P13866; B2R7E2; B7Z4Q9; B7ZA69
• Molecular Weight: 60,097 Da
• NCBI Official Full Name: sodium/glucose cotransporter 1 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 5 member 1
• NCBI Official Symbol: SLC5A1
• NCBI Official Synonym Symbols: NAGT; SGLT1; D22S675
• NCBI Protein Information: sodium/glucose cotransporter 1
• UniProt Protein Name: Sodium/glucose cotransporter 1
• UniProt Gene Name: SLC5A1
• UniProt Synonym Gene Names: NAGT; SGLT1; Na(+)/glucose cotransporter 1

NCBI Description

This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Actively transports glucose into cells by Na+ cotransport with a Na+ to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na+/glucose cotransporter arranged in series along kidney proximal tubules.