Human MutY Homolog (MUTYH) RTU ELISA Kit | MUTYH rtu elisa kit

Human MutY Homolog (MUTYH) ELISA Kit

Cat. Num. AAA4501221

• Gene Names: MUTYH; MYH
• Reactivity: Human
• Synonyms: MutY Homolog (MUTYH); Human MutY Homolog (MUTYH) ELISA Kit; MYH; A/G-specific adenine DNA glycosylase; MUTYH rtu elisa kit

• Reactivity: Human
• Sequence Length: 535

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.041ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUTYH rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUTYH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MUTYH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUTYH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MUTYH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115298648
• NCBI GeneID: 4595
• NCBI Accession #: NP_001041636.1
• NCBI GenBank Nucleotide #: NM_001048171.1
• UniProt Accession #: Q9UIF7; Q15830; Q9UBP2; Q9UBS7; Q9UIF4; Q9UIF5; Q9UIF6; D3DPZ4
• Molecular Weight: 57,446 Da
• NCBI Official Full Name: adenine DNA glycosylase isoform 2
• NCBI Official Synonym Full Names: mutY DNA glycosylase
• NCBI Official Symbol: MUTYH
• NCBI Official Synonym Symbols: MYH
• NCBI Protein Information: adenine DNA glycosylase
• UniProt Protein Name: Adenine DNA glycosylase
• UniProt Gene Name: MUTYH
• UniProt Synonym Gene Names: MYH; hMYH

NCBI Description

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]

Uniprot Description

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.