Human Lactoperoxidase (LPO) RTU ELISA Kit | LPO rtu elisa kit

Human Lactoperoxidase (LPO) ELISA Kit

Cat. Num. AAA4500832

• Gene Names: LPO; SPO
• Reactivity: Human
• Synonyms: Lactoperoxidase (LPO); Human Lactoperoxidase (LPO) ELISA Kit; SPO; Salivary peroxidase; LPO rtu elisa kit

• Reactivity: Human
• Sequence Length: 629

• Assay Type: Sandwich
• Samples: Breast Milk, Tears or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LPO rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LPO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 231569458
• NCBI GeneID: 4025
• NCBI Accession #: NP_001153574.1
• NCBI GenBank Nucleotide #: NM_001160102.1
• UniProt Accession #: P22079; Q13408; Q3KNQ2; A5JUY4; E7EMJ3
• Molecular Weight: 70,984 Da
• NCBI Official Full Name: lactoperoxidase isoform 3 preproprotein
• NCBI Official Synonym Full Names: lactoperoxidase
• NCBI Official Symbol: LPO
• NCBI Official Synonym Symbols: SPO
• NCBI Protein Information: lactoperoxidase
• UniProt Protein Name: Lactoperoxidase
• Protein Family: Lactoperoxidase
• UniProt Gene Name: LPO
• UniProt Synonym Gene Names: SAPX; LPO; SPO

NCBI Description

This gene encodes a member of the peroxidase family of proteins. The encoded preproprotein is proteolytically processed to generate the mature enzyme. Following its secretion from salivary, mammary, and other mucosal glands, this enzyme catalyzes the generation of the antimicrobial substance hypothiocyanous acid. This gene is present in a gene cluster on chromosome 17. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN) (). May contribute to airway host defense against infection.