Human Gastrin Releasing Peptide (GRP) RTU ELISA Kit | GRP rtu elisa kit

Human Gastrin Releasing Peptide (GRP) ELISA Kit

Cat. Num. AAA459727

• Gene Names: GRP; BN; GRP-10; proGRP; preproGRP
• Reactivity: Human
• Synonyms: Gastrin Releasing Peptide (GRP); Human Gastrin Releasing Peptide (GRP) ELISA Kit; BN; GRP10; Bombesin; Neuromedin C; GRP rtu elisa kit

• Reactivity: Human
• Sequence Length: 141

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 4.64pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Related Product Information for GRP rtu elisa kit

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GRP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GRP and unlabeled GRP (Standards or samples) with the pre-coated antibody specific to GRP. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GRP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GRP in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 60498997
• NCBI GeneID: 2922
• NCBI Accession #: NP_001012530.1
• NCBI GenBank Nucleotide #: NM_001012512.2
• UniProt Accession #: P07492; P07491; P81553; Q14454; Q53YA0; Q9BSY7
• Molecular Weight: 15,533 Da
• NCBI Official Full Name: gastrin-releasing peptide isoform 2
• NCBI Official Synonym Full Names: gastrin releasing peptide
• NCBI Official Symbol: GRP
• NCBI Official Synonym Symbols: BN; GRP-10; proGRP; preproGRP
• NCBI Protein Information: gastrin-releasing peptide
• UniProt Protein Name: Gastrin-releasing peptide
• Protein Family: Gastrin-releasing peptide
• UniProt Gene Name: GRP
• UniProt Synonym Gene Names: GRP

NCBI Description

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. The encoded preproprotein is proteolytically processed to generate two peptides, gastrin-releasing peptide and neuromedin-C. These peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

Stimulates the release of gastrin and other gastrointestinal hormones (). Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. Contributes to long-term fear memory, but not normal spatial memory. Contributes to the regulation of food intake ().