Human Flightless I Homolog (FLII) RTU ELISA Kit | FLII rtu elisa kit
Human Flightless I Homolog (FLII) ELISA Kit
Gene Names
FLII; FLI; FLIL; Fli1
Reactivity
Human
Synonyms
Flightless I Homolog (FLII); Human Flightless I Homolog (FLII) ELISA Kit; FLI; FLIL; Fli1; FLII rtu elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of FLII. No significant cross-reactivity or interference between FLII and analogues was observed.
Sequence Length
1258
Samples
Human tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.1-5000pg/mL
Sensitivity
< 29.2pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FLII were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FLII were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FLII rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FLII in human tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FLII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FLII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FLII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FLII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FLII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FLII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FLII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FLII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
143,652 Da
NCBI Official Full Name
protein flightless-1 homolog isoform 2
NCBI Official Synonym Full Names
FLII, actin remodeling protein
NCBI Official Symbol
FLII
NCBI Official Synonym Symbols
FLI; FLIL; Fli1
NCBI Protein Information
protein flightless-1 homolog
UniProt Protein Name
Protein flightless-1 homolog
Protein Family
UniProt Gene Name
FLII
UniProt Synonym Gene Names
FLIL
NCBI Description
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Uniprot Description
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
