Human A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7 ELISA Kit | ADAMTS7 elisa kit

Human A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7 ELISA Kit

Cat. Num. AAA732016

• Gene Names: ADAMTS7; ADAM-TS7; ADAMTS-7; ADAM-TS 7
• Reactivity: Human
• Synonyms: A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7; Human A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7 ELISA Kit; ADAMTS7 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 250-5000 pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS7 elisa kit

Intended Uses: This ADAMTS7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTS7. This ELISA kit for research use only!

Principle of the Assay: ADAMTS7 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADAMTS7. Standards or samples are then added to the microtiter plate wells and ADAMTS7 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADAMTS7 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADAMTS7 are added to each well to "sandwich" the ADAMTS7 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADAMTS7 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADAMTS7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 38683827
• NCBI GeneID: 11173
• NCBI Accession #: NP_055087.2
• NCBI GenBank Nucleotide #: NM_014272.3
• Molecular Weight: 184,095 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 7 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 7
• NCBI Official Symbol: ADAMTS7
• NCBI Official Synonym Symbols: ADAM-TS7; ADAMTS-7; ADAM-TS 7
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; a disintegrin and metalloprotease with thrombospondin motifs-7 preproprotein; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 7
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS7
• UniProt Synonym Gene Names: ADAM-TS 7; ADAM-TS7; ADAMTS-7
• UniProt Entry Name: ATS7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two C-terminal TS motifs. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS7: Metalloprotease that may play a role in the degradation of COMP.

Protein type: EC 3.4.24.-; Motility/polarity/chemotaxis; Secreted, signal peptide; Protease; Cell surface; Secreted

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen

Molecular Function: protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; post-translational protein modification