Human Fatty Acid Synthase (FASN) RTU ELISA Kit | FASN rtu elisa kit

Human Fatty Acid Synthase (FASN) ELISA Kit

Cat. Num. AAA459203

• Gene Names: FASN; FAS; OA-519; SDR27X1
• Reactivity: Human
• Synonyms: Fatty Acid Synthase (FASN); Human Fatty Acid Synthase (FASN) ELISA Kit; FAS; OA519; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X; Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; FASN rtu elisa kit

• Reactivity: Human
• Sequence Length: 2511

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.124ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FASN rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FASN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FASN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FASN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FASN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 41872631
• NCBI GeneID: 2194
• NCBI Accession #: NP_004095.4
• NCBI GenBank Nucleotide #: NM_004104.4
• UniProt Accession #: P49327; Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
• Molecular Weight: 273,427 Da
• NCBI Official Full Name: fatty acid synthase
• NCBI Official Synonym Full Names: fatty acid synthase
• NCBI Official Symbol: FASN
• NCBI Official Synonym Symbols: FAS; OA-519; SDR27X1
• NCBI Protein Information: fatty acid synthase
• UniProt Protein Name: Fatty acid synthase
• Protein Family: Fatty acid synthase
• UniProt Gene Name: FASN
• UniProt Synonym Gene Names: FAS

NCBI Description

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

Uniprot Description

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.