Human Dopamine Beta Hydroxylase (DbH) RTU ELISA Kit | DbH rtu elisa kit

Human Dopamine Beta Hydroxylase (DbH) ELISA Kit

Cat. Num. AAA458784

• Gene Names: DBH; DBM
• Reactivity: Human
• Synonyms: Dopamine Beta Hydroxylase (DbH); Human Dopamine Beta Hydroxylase (DbH) ELISA Kit; DBM; Dopamine Beta-Monooxygenase; DbH rtu elisa kit

• Reactivity: Human
• Sequence Length: 617

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.24ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DbH rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DbH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DbH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DbH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DbH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116534900
• NCBI GeneID: 1621
• NCBI Accession #: NP_000778.3
• NCBI GenBank Nucleotide #: NM_000787.3
• UniProt Accession #: P09172; Q5T381; Q96AG2
• Molecular Weight: 69,065 Da
• NCBI Official Full Name: dopamine beta-hydroxylase
• NCBI Official Synonym Full Names: dopamine beta-hydroxylase
• NCBI Official Symbol: DBH
• NCBI Official Synonym Symbols: DBM
• NCBI Protein Information: dopamine beta-hydroxylase
• UniProt Protein Name: Dopamine beta-hydroxylase
• Protein Family: Dopamine beta-hydroxylase
• UniProt Gene Name: DBH

NCBI Description

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]

Uniprot Description

Conversion of dopamine to noradrenaline.