Human Dimethylarginine Dimethylaminohydrolase 1 (DDAH1) RTU ELISA Kit | DDAH1 rtu elisa kit

Human Dimethylarginine Dimethylaminohydrolase 1 (DDAH1) ELISA Kit

Cat. Num. AAA458802

• Gene Names: DDAH1; DDAH; DDAHI; DDAH-1; HEL-S-16
• Reactivity: Human
• Synonyms: Dimethylarginine Dimethylaminohydrolase 1 (DDAH1); Human Dimethylarginine Dimethylaminohydrolase 1 (DDAH1) ELISA Kit; DDAH; Dimethylargininase-1; N(G); N(G)-dimethylarginine dimethylaminohydrolase 1; DDAH1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 182

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DDAH1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDAH1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DDAH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DDAH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DDAH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 197313763
• NCBI GeneID: 23576
• NCBI Accession #: NP_001127917.1
• NCBI GenBank Nucleotide #: NM_001134445.1
• UniProt Accession #: O94760; Q5HYC8; Q86XK5
• Molecular Weight: 20,189 Da
• NCBI Official Full Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform 2
• NCBI Official Synonym Full Names: dimethylarginine dimethylaminohydrolase 1
• NCBI Official Symbol: DDAH1
• NCBI Official Synonym Symbols: DDAH; DDAHI; DDAH-1; HEL-S-16
• NCBI Protein Information: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
• UniProt Protein Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
• Protein Family: N(G),N(G)-dimethylarginine dimethylaminohydrolase
• UniProt Gene Name: DDAH1
• UniProt Synonym Gene Names: DDAH; DDAH-1; Dimethylarginine dimethylaminohydrolase 1

NCBI Description

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.