Mouse Nuclear receptor corepressor 1 ELISA Kit | Ncor1 elisa kit

Mouse Nuclear receptor corepressor 1 ELISA Kit

Cat. Num. AAA765429

• Gene Names: Ncor1; N-CoR; RIP13; Rxrip13; mKIAA1047; 5730405M06Rik; A230020K14Rik
• Reactivity: Mouse
• Synonyms: Nuclear receptor corepressor 1; Mouse Nuclear receptor corepressor 1 ELISA Kit; Ncor1/KIAA1047/N-CoR/TRAC1/hN-CoR/KIAA1047N-CoR1/MGC104216/N-CoRhCIT529I10/nuclear receptor corepressor 1/nuclear receptor co-repressor 1/thyroid hormone- and retinoic acid receptor-associated corepressor 1/TRAC1; Ncor1 elisa kit

• Reactivity: Mouse
• Sequence Length: 2454

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ncor1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ncor1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ncor1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ncor1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ncor1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 356640155
• NCBI GeneID: 20185
• NCBI Accession #: NP_001239242.1
• NCBI GenBank Nucleotide #: NM_001252313.1
• Molecular Weight: 266,519 Da
• NCBI Official Full Name: nuclear receptor corepressor 1 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor co-repressor 1
• NCBI Official Symbol: Ncor1
• NCBI Official Synonym Symbols: N-CoR; RIP13; Rxrip13; mKIAA1047; 5730405M06Rik; A230020K14Rik
• NCBI Protein Information: nuclear receptor corepressor 1
• UniProt Protein Name: Nuclear receptor corepressor 1
• Protein Family: Nuclear receptor corepressor
• UniProt Gene Name: Ncor1
• UniProt Synonym Gene Names: Rxrip13; N-CoR; N-CoR1; RIP13
• UniProt Entry Name: NCOR1_MOUSE

Uniprot Description

N-CoR1: is a protein that regulates the activity of some transcription factors, including nuclear receptors, by promoting histone deacetylation and chromatin condensation, thereby altering the accessibility of various genes to the transcriptional machinery. Is known to play a role genetic programming including that for myogenesis. Is a component of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and the retinoic acid receptors in the absence of ligand. Interacts with the catalytic domain of HDAC9. Increased expression correlates with the loss of vitamin D responsiveness in aggressive androgen-independent prostate cancer cells. Its gene tends to be overexpressed in multiple-myeloma cell lines.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: cytoplasm; histone deacetylase complex; membrane; nuclear chromatin; nucleus; perinuclear region of cytoplasm; Sin3 complex; spindle microtubule; transcription factor complex; transcriptional repressor complex

Molecular Function: chromatin binding; DNA binding; estrogen receptor binding; histone deacetylase binding; histone deacetylase regulator activity; ligand-dependent nuclear receptor binding; nuclear hormone receptor binding; peroxisome proliferator activated receptor binding; protein binding; protein domain specific binding; retinoic acid receptor binding; retinoid X receptor binding; sequence-specific DNA binding; thyroid hormone receptor binding; transcription corepressor activity; transcription factor activity

Biological Process: CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; cholesterol homeostasis; circadian regulation of gene expression; negative regulation of JNK cascade; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of histone deacetylation; regulation of multicellular organism growth; regulation of transcription from RNA polymerase II promoter; spindle assembly; thalamus development