Human Dentin Sialophosphoprotein (DSPP) RTU ELISA Kit | DSPP rtu elisa kit

Human Dentin Sialophosphoprotein (DSPP) ELISA Kit

Cat. Num. AAA458939

• Gene Names: DSPP; DPP; DSP; DGI1; DMP3; DFNA39
• Reactivity: Human
• Synonyms: Dentin Sialophosphoprotein (DSPP); Human Dentin Sialophosphoprotein (DSPP) ELISA Kit; DSP; DFNA39; DGI1; DPP; DTDP2; DMP3; Dentin phosphoprotein; Dentin phosphophoryn; Dentin sialoprotein; DSPP rtu elisa kit

• Reactivity: Human
• Sequence Length: 1301

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.55ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DSPP rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DSPP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DSPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DSPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DSPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 89001107
• NCBI GeneID: 1834
• NCBI Accession #: NP_055023.2
• NCBI GenBank Nucleotide #: NM_014208.3
• UniProt Accession #: Q9NZW4; O95815; A8MUI0
• Molecular Weight: 131,151 Da
• NCBI Official Full Name: dentin sialophosphoprotein preproprotein
• NCBI Official Synonym Full Names: dentin sialophosphoprotein
• NCBI Official Symbol: DSPP
• NCBI Official Synonym Symbols: DPP; DSP; DGI1; DMP3; DFNA39
• NCBI Protein Information: dentin sialophosphoprotein
• UniProt Protein Name: Dentin sialophosphoprotein
• Protein Family: Dentin sialophosphoprotein
• UniProt Gene Name: DSPP
• UniProt Synonym Gene Names: DPP; DSP

NCBI Description

This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mutations in this gene are associated with dentinogenesis imperfecta and dentin dysplasia. This gene is present in a gene cluster on chromosome 4. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.