Human Coagulation Factor XII (F12) RTU ELISA Kit | F12 rtu elisa kit

Human Coagulation Factor XII (F12) ELISA Kit

Cat. Num. AAA459139

• Gene Names: F12; HAF; HAE3; HAEX
• Reactivity: Human
• Synonyms: Coagulation Factor XII (F12); Human Coagulation Factor XII (F12) ELISA Kit; FXII; HAF; Contact Factor; Hageman Factor; F12 rtu elisa kit

• Reactivity: Human
• Sequence Length: 615

• Assay Type: Sandwich
• Samples: Plasma.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.065ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F12 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 145275213
• NCBI GeneID: 2161
• NCBI Accession #: NP_000496.2
• NCBI GenBank Nucleotide #: NM_000505.3
• UniProt Accession #: P00748; P78339
• Molecular Weight: 67,792 Da
• NCBI Official Full Name: coagulation factor XII preproprotein
• NCBI Official Synonym Full Names: coagulation factor XII
• NCBI Official Symbol: F12
• NCBI Official Synonym Symbols: HAF; HAE3; HAEX
• NCBI Protein Information: coagulation factor XII
• UniProt Protein Name: Coagulation factor XII
• Protein Family: Coagulation factor
• UniProt Gene Name: F12
• UniProt Synonym Gene Names: HAF

NCBI Description

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]

Uniprot Description

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.