Rat Coagulation Factor XII ELISA Kit | FXII elisa kit

Rat Coagulation Factor XII ELISA Kit

Cat. Num. AAA760086

• Gene Names: F12; HAF; HAE3; HAEX
• Reactivity: Rat
• Synonyms: Coagulation Factor XII; Rat Coagulation Factor XII ELISA Kit; FXII/F12 (Coagulation factor XII)/HAEX/Hageman factor/HAE3/HAF; FXII elisa kit

• Reactivity: Rat
• Sequence Length: 612

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FXII elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat FXII antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat FXII antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat FXII amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat FXII can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 182292
• NCBI GeneID: 2161
• Molecular Weight: 67,792 Da
• NCBI Official Full Name: coagulation factor XII, partial
• NCBI Official Synonym Full Names: coagulation factor XII
• NCBI Official Symbol: F12
• NCBI Official Synonym Symbols: HAF; HAE3; HAEX
• NCBI Protein Information: coagulation factor XII
• UniProt Protein Name: Coagulation factor XII
• Protein Family: Coagulation factor
• UniProt Gene Name: F12
• UniProt Synonym Gene Names: HAF
• UniProt Entry Name: FA12_HUMAN

NCBI Description

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]

Uniprot Description

F12: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta- factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Defects in F12 are the cause of factor XII deficiency (FA12D); also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3); also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. Belongs to the peptidase S1 family.

Protein type: Chaperone; Secreted; EC 3.4.21.38; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: misfolded protein binding; protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation; fibrinolysis; innate immune response; plasma kallikrein-kinin cascade; positive regulation of blood coagulation; positive regulation of fibrinolysis; protein autoprocessing; protein processing; response to misfolded protein; zymogen activation

Disease: Angioedema, Hereditary, Type Iii; Factor Xii Deficiency