Human Coagulation Factor XI (F11) RTU ELISA Kit | F11 rtu elisa kit
Human Coagulation Factor XI (F11) ELISA Kit
Gene Names
F11; FXI; PTA
Reactivity
Human
Synonyms
Coagulation Factor XI (F11); Human Coagulation Factor XI (F11) ELISA Kit; FXI; PTA; Fletcher Factor; Prekallikrein; Plasma Thromboplastin Antecedent; F11 rtu elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of F11. No significant cross-reactivity or interference between F11 and analogues was observed.
Sequence Length
625
Samples
Human plasma or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.127ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F11 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F11 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for F11 rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of F11 in human plasma or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to F11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,840 Da
NCBI Official Full Name
coagulation factor XI isoform 1 preproprotein
NCBI Official Synonym Full Names
coagulation factor XI
NCBI Official Symbol
F11
NCBI Official Synonym Symbols
FXI; PTA
NCBI Protein Information
coagulation factor XI
UniProt Protein Name
Coagulation factor XI
Protein Family
UniProt Gene Name
F11
UniProt Synonym Gene Names
FXI; PTA
NCBI Description
This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality. [provided by RefSeq, Jul 2008]
Uniprot Description
Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
