Human Chemokine C-C-Motif Ligand 14 (CCL14) RTU ELISA Kit | CCL14 rtu elisa kit

Human Chemokine C-C-Motif Ligand 14 (CCL14) ELISA Kit

Cat. Num. AAA458190

• Gene Names: CCL14; CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• Reactivity: Human
• Synonyms: Chemokine C-C-Motif Ligand 14 (CCL14); Human Chemokine C-C-Motif Ligand 14 (CCL14) ELISA Kit; SCYA14; HCC-1; HCC-3; NCC-2; SCYL2; CKb1; MCIF; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 14; CCL14 rtu elisa kit

• Reactivity: Human
• Sequence Length: 109

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 6.8pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL14 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCL14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CCL14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCL14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCL14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14589959
• NCBI GeneID: 6358
• NCBI Accession #: NP_116738.1
• NCBI GenBank Nucleotide #: NM_032962.4
• UniProt Accession #: Q16627; Q13954; E1P649; E1P650
• Molecular Weight: 12,297 Da
• NCBI Official Full Name: C-C motif chemokine 14 isoform 2
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 14
• NCBI Official Symbol: CCL14
• NCBI Official Synonym Symbols: CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• NCBI Protein Information: C-C motif chemokine 14
• UniProt Protein Name: C-C motif chemokine 14
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL14
• UniProt Synonym Gene Names: NCC2; SCYA14; HCC-1/HCC-3

NCBI Description

This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]

Uniprot Description

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.