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Typical Testing Data/Standard Curve (for reference only)

Human Caldesmon (CALD) RTU ELISA Kit | CALD rtu elisa kit

Human Caldesmon (CALD) ELISA Kit

Gene Names
CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
Reactivity
Human
Synonyms
Caldesmon (CALD); Human Caldesmon (CALD) ELISA Kit; CALD1; CDM; H-CAD; L-CAD; NAG22; CALD rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
538
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.781-50ng/mL
Sensitivity
0.25ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CALD rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CALD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CALD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CALD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CALD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
800
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,620 Da
NCBI Official Full Name
caldesmon isoform 2
NCBI Official Synonym Full Names
caldesmon 1
NCBI Official Symbol
CALD1
NCBI Official Synonym Symbols
CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
NCBI Protein Information
caldesmon
UniProt Protein Name
Caldesmon
UniProt Gene Name
CALD1
UniProt Synonym Gene Names
CAD; CDM; CDM

NCBI Description

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration ().

Research Articles on CALD

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Product Notes

The Human CALD cald1 (Catalog #AAA458083) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA458083 RTU ELISA Kit recognizes Human CALD. It is sometimes possible for the material contained within the vial of "Caldesmon (CALD), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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