Rabbit DDX60 Polyclonal Antibody | anti-DDX60 antibody

DDX60 Antibody

Cat. Num. AAA153369

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: DDX60 Antibody is affinity chromatography purified via peptide column.
• Synonyms: DDX60; Polyclonal Antibody; DDX60 Antibody; Probable ATP-dependent RNA helicase DDX60; DEAD box protein 60; DEAD (Asp-Glu-Ala-Asp) box polypeptide 60; anti-DDX60 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.
• Purity/Purification: DDX60 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1712

• Applicable Applications for anti-DDX60 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.
• Buffer: DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DDX60 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DDX60 in A20 cell lysate with DDX60 antibody at (A) 1 and (B) 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DDX60 in A20 cells with DDX60 antibody at 20 μg/mL.)

Related Product Information for anti-DDX60 antibody

DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 222831595
• NCBI GeneID: 55601
• NCBI Accession #: NP_060101
• NCBI GenBank Nucleotide #: NM_017631.5
• UniProt Accession #: Q8IY21; Q6PK35; Q9NVE3
• Molecular Weight: 188 kDa
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX60
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
• NCBI Official Symbol: DDX60
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX60; DEAD box protein 60
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX60
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX60
• UniProt Entry Name: DDX60_HUMAN

Uniprot Description

DDX60: Positively regulates DDX58/RIG-I- and IFIH1/MDA5- dependent type I interferon and interferon inducible gene expression in response to viral infection. Binds ssRNA, dsRNA and dsDNA and can promote the binding of DDX58/RIG-I to dsRNA. Exhibits antiviral activity against hepatitis C virus and vesicular stomatitis virus (VSV). Belongs to the helicase family.

Protein type: EC 3.6.4.13; Hydrolase

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: intermediate filament cytoskeleton; cytoplasm

Molecular Function: protein binding; single-stranded RNA binding; double-stranded DNA binding; double-stranded RNA binding; helicase activity; ATP binding

Biological Process: metabolic process; response to virus; innate immune response; defense response to virus