Human Alpha-2-Microglobulin Like Protein 1 (a2ML1) RTU ELISA Kit | a2ML1 rtu elisa kit

Human Alpha-2-Microglobulin Like Protein 1 (a2ML1) ELISA Kit

Cat. Num. AAA457274

• Gene Names: A2ML1; p170; CPAMD9
• Reactivity: Human
• Synonyms: Alpha-2-Microglobulin Like Protein 1 (a2ML1); Human Alpha-2-Microglobulin Like Protein 1 (a2ML1) ELISA Kit; CPAMD9; C3 And PZP-Like; Alpha-2-Macroglobulin Domain Containing 9; a2ML1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 963

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for a2ML1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to a2ML1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to a2ML1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain a2ML1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of a2ML1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 539846446
• NCBI GeneID: 144568
• NCBI Accession #: NP_001269353.1
• NCBI GenBank Nucleotide #: NM_001282424.2
• UniProt Accession #: A8K2U0; Q2M224; Q6ZW52; Q6ZW53; Q8N1M4; Q96LQ8; B5MDD1; B7Z7V4; D3DUV3; F5H2Z2
• Molecular Weight: 105,968 Da
• NCBI Official Full Name: alpha-2-macroglobulin-like protein 1 isoform 2
• NCBI Official Synonym Full Names: alpha-2-macroglobulin like 1
• NCBI Official Symbol: A2ML1
• NCBI Official Synonym Symbols: p170; CPAMD9
• NCBI Protein Information: alpha-2-macroglobulin-like protein 1
• UniProt Protein Name: Alpha-2-macroglobulin-like protein 1
• Protein Family: Alpha-2-macroglobulin-like protein
• UniProt Gene Name: A2ML1

NCBI Description

This gene encodes a member of the alpha-macroglobulin superfamily. The encoded protein is thought to be an N-glycosylated monomeric protein that acts as an inhibitor of several proteases. It has been shown to form covalent interactions with proteases, and has been reported as the p170 antigen recognized by autoantibodies in the autoimmune disease paraneoplastic pemphigus (PNP; PMID:20805888). Mutations in these gene have also been associated with some cases of Noonan syndrome (NS; PMID:24939586) as well as some cases of otitis media (PMID:26121085). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.