Matrix Metalloproteinase, Pro, 13 Active Protein | MMP14 active protein

Matrix Metalloproteinase, Pro, 13, Recombinant (ProMMP-13, Procollagenase III)

Cat. Num. AAA637205

• Gene Names: MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; MT1-MMP; MT-MMP 1
• Purity: Highly Purified; Pro-MMP-13 appears as a major band at about 60kD in SDS-PAGE (> 95% total protein).
• Synonyms: Matrix Metalloproteinase; Pro; 13; Recombinant (ProMMP-13; Procollagenase III); MMP14 active protein

• Host: Human recombinant from E Coli
• Purity/Purification: Highly Purified; Pro-MMP-13 appears as a major band at about 60kD in SDS-PAGE (> 95% total protein).
• Form/Format: Provided as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 5mM CaCl2, 0.05% Brij-35.

• Specific Activity: The specific activity of activated MMP-13 is 250-300mU/mg, where 1U is the activity that hydrolyzes 1umol peptide (7-methoxycoumarin-4-yl)acetyl-Pro- Leu-Gly-Leu-Dpa-Ala-Arg) within 1 minute under the assay conditions described by Knight,et al. ACTIVATION: An aliquot of 19.5ul procollagenase-3 is mixed with 0.5ul APMA solution (40mM p-aminophenyl mercuric acetate in DMSO) and the mixture is incubated for 30 minutes at 37 degree C. The mixture may be stored on ice until use for activity assays.
• Preparation and Storage: Maintain frozen at-70 degree C in undiluted aliquots. The enzyme may be stored at -20 degree C for several weeks without significant loss of activity. Repeated freezing and thawing should be avoided.

Related Product Information for MMP14 active protein

Matrix metalloproteinases (MMPs) are Zn 2+-and Ca 2+-dependent endopeptidases which function in the turnover of extracellular matrix components (1). Main subfamilies of MMP are collagenases, gelatinases, stromelysins and membrane-type matrix metalloproteinases (2). Three homologous collagenases have been identified in human tissues: Interstitial collagenase, neutrophil collagenase and collagenase-3. These three enzymes cleave fibrillar collagens at a single site, generating fragments of approximately 3/4 and 1/4 the size of the original molecules. ProMMP-13 (Procollagenase-3) consists of 452 amino acids with a calculated Mr of 52.520(3). Due to N-linked glycosylation, the actual Mr is about 60000 Da (4). Within the protein the following domains and sequence regions can be distinguished (3,4): An N-terminal propeptide, which confers latency to the proenzyme, a Ca2+ and Zn2+-ion binding catalytic domain, a hinge region, and a C-terminal hemopexin-like domain. Latent procollagenase-3 can be activated by proteases such as stromelysin (4), gelatinase A, MT1-MMP and plasmin (5) or incubation with APMA (4). The Mr of active collagenase-3 which begins with the N-terminal sequence YNVFPRTL is 48,000 Da. Collagenase-3 hydrolyzes type II collagen 5-to 6-times faster than type I and type III collagens. The enzyme also exhibits high activity towards gelatin and it degrades SERPINS as a1-antichymotrypsin and plasminogen activator inhibitor-2 (4). Collagenase-3 is inhibited in a 1:1 stoichiometric fashion by TIMP-1, TIMP-2 and TIMP-3. Collagenase-3 is expressed during fetal bone development (6). In adult human tissues collagenase-3 has been detected only in pathological conditions: in malignant tumors (3), in chronic ulcers (7), in arthritic cartilage (8) and synovium (9). The recombinant catalytic domain of Pro-MMP-13 is useful for the study of degradation of extracellular matrix proteins.. The enzyme allows screening of matrix metalloproteinase inhibitors and characterization of inhibitor action.

Recombinant 452 amino acid polypeptide corresponding to human Pro-MMP-13, with an additional C-terminal His-tag with the sequence GVTHHHHHH expressed in E. coli and purified from periplasm. The calculated Mr is 51.681 Da. Upon activation with APMA activated MMP-13 is formed.

Product Categories/Family for MMP14 active protein

Molecular Biology; MB-Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 793763
• NCBI GeneID: 4323
• UniProt Accession #: P50281; Q6GSF3; Q92678; A8K5L0
• Molecular Weight: 65,894 Da
• NCBI Official Full Name: membrane-type matrix metalloproteinase
• NCBI Official Synonym Full Names: matrix metallopeptidase 14 (membrane-inserted)
• NCBI Official Symbol: MMP14
• NCBI Official Synonym Symbols: MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; MT1-MMP; MT-MMP 1
• NCBI Protein Information: matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase
• UniProt Protein Name: Matrix metalloproteinase-14
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP14
• UniProt Synonym Gene Names: MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP
• UniProt Entry Name: MMP14_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.

Protein type: Protease; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.80

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; plasma membrane; melanosome

Molecular Function: integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity

Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; negative regulation of focal adhesion formation; male gonad development; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; extracellular matrix disassembly; collagen catabolic process; branching morphogenesis of a tube; ovarian follicle development; regulation of transcription, DNA-dependent; response to mechanical stimulus; response to estrogen stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; response to oxidative stress; angiogenesis; astrocyte cell migration; positive regulation of cell migration; endochondral ossification; lung development

Disease: Winchester Syndrome