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Western Blot (WB) (Western blot analysis of extracts from 293 cells, using DNA Polymerase Ž» antibody.)

Rabbit DNA Polymerase lambda Polyclonal Antibody | anti-POLL antibody

DNA Polymerase lambda Antibody

Gene Names
POLL; BETAN; POLKAPPA
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DNA Polymerase lambda; Polyclonal Antibody; DNA Polymerase lambda Antibody; DNA polymerase lambda; EC 2.7.7.7; EC 4.2.99.-; Pol Lambda; DNA polymerase kappa; anti-POLL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total DNA Polymerase lambda protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
575
Applicable Applications for anti-POLL antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human DNA Polymerase lambda.
Target Name
DNA Polymerase lambda
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, using DNA Polymerase Ž» antibody.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells, using DNA Polymerase Ž» antibody.)
Related Product Information for anti-POLL antibody
Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Product Categories/Family for anti-POLL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,839 Da
NCBI Official Full Name
DNA polymerase lambda isoform a
NCBI Official Synonym Full Names
polymerase (DNA directed), lambda
NCBI Official Symbol
POLL
NCBI Official Synonym Symbols
BETAN; POLKAPPA
NCBI Protein Information
DNA polymerase lambda
UniProt Protein Name
DNA polymerase lambda
Protein Family
UniProt Gene Name
POLL
UniProt Synonym Gene Names
Pol Lambda; Pol beta2
UniProt Entry Name
DPOLL_HUMAN

NCBI Description

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.

Protein type: EC 2.7.7.7; Lyase; Transferase; DNA repair, damage

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; DNA-directed DNA polymerase activity; lyase activity

Biological Process: nucleotide-excision repair; somatic hypermutation of immunoglobulin genes; DNA replication

Research Articles on POLL

Similar Products

Product Notes

The POLL poll (Catalog #AAA9414258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNA Polymerase lambda Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Polymerase lambda can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the POLL poll for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Polymerase lambda, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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