Matrix Metalloproteinase 17 MT4 Catalytic Domain Active Protein | MMP17 active protein

Matrix Metalloproteinase 17 MT4, Recombinant Catalytic Domain (MT4-MMP-17)

Cat. Num. AAA635612

• Gene Names: MMP17; MT4-MMP
• Purity: Purified; MT4-MMP catalytic domain appears as a homogeneous band at 24kD in SDS-PAGE
• Synonyms: Matrix Metalloproteinase 17 MT4 Catalytic Domain; Matrix Metalloproteinase 17 MT4; Recombinant Catalytic Domain (MT4-MMP-17); MMP17 active protein

• Host: Human recombinant from E Coli
• Purity/Purification: Purified; MT4-MMP catalytic domain appears as a homogeneous band at 24kD in SDS-PAGE
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 5mM calcium chloride, 0.1% Triton X-100. No preservatives added.

• Specific Activity: The specific activity of MT4-MMP catalytic domain is 3-6mU/mg, where 1U is the activity that hydrolyzes 1umol peptide (7-methoxycoumarin- 4-yl)acetyl-Pro- Leu-Gly-Leu-Dpa-Ala-; Arg) within 1 minute under the assay conditions described by Knight,et al.
• Preparation and Storage: May be stored at 4 degree C for 1 week or at -20 degree C for several weeks without significant loss of activity. For long-term storage, aliquot and store at -70 degree C. Aliquots are stable for at least 6 months at -70 degree C. Repeated freezing and thawing should be avoided. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for MMP17 active protein

Matrix metalloproteinases (MMPs) are Zn2+- and Ca2+-dependent endopeptidases which function in the turnover of extracellular matrix components (1). Presently, eighteen secreted MMPs and five membrane-

Recombinant 186aa polypeptide, produced by tryptic activation of a recombinant soluble proform purified from E. coli periplasm. The polypeptide contains the native sequence of 185aa, plus a C-terminal linked polyhistidine tag. The calculated Mr of recombinant catalytic domain is 21,693D.

Product Categories/Family for MMP17 active protein

Molecular Biology; MB-Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 112382270
• NCBI GeneID: 4326
• NCBI Accession #: NP_057239.4
• NCBI GenBank Nucleotide #: NM_016155.4
• UniProt Accession #: Q9ULZ9; Q14850
• Molecular Weight: 66,653 Da
• NCBI Official Full Name: matrix metalloproteinase-17 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 17 (membrane-inserted)
• NCBI Official Symbol: MMP17
• NCBI Official Synonym Symbols: MT4-MMP
• NCBI Protein Information: matrix metalloproteinase-17; MMP-17; MT4MMP; MTMMP4; MT-MMP 4; membrane-type matrix metalloproteinase 4; membrane-type-4 matrix metalloproteinase; matrix metalloproteinase 17 (membrane-inserted)
• UniProt Protein Name: Matrix metalloproteinase-17
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP17
• UniProt Synonym Gene Names: MT4MMP; MMP-17; MT-MMP 4; MTMMP4; MT4-MMP; MT4MMP
• UniProt Entry Name: MMP17_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene is considered a member of the membrane-type MMP (MT-MMP) subfamily. However, this protein is unique among the MT-MMP's in that it is a GPI-anchored protein rather than a transmembrane protein. The protein activates MMP-2 by cleavage. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP17: Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Protease; Activator; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: proteinaceous extracellular matrix; integral to plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; proteolysis