Human Matrix Metalloproteinase 17 (MMP17) ELISA Kit | MMP17 elisa kit

Human Matrix Metalloproteinase 17 (MMP17) ELISA Kit

Cat. Num. AAA2000010

• Gene Names: MMP17; MT4-MMP
• Reactivity: Human
• Synonyms: Matrix Metalloproteinase 17 (MMP17); Human Matrix Metalloproteinase 17 (MMP17) ELISA Kit; MT4-MMP; MMP4; Membrane-type matrix metalloproteinase 4; Membrane-type-4 matrix metalloproteinase; MMP17 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 17 (MMP17).; No significant cross-reactivity or interference between Matrix Metalloproteinase 17 (MMP17) and analogues was observed.
• Sequence Length: 603

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 17 (MMP17) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 17 (MMP17) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP17 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 17 (MMP17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 17 (MMP17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 17 (MMP17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 17 (MMP17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MMP17 elisa kit

Enzyme & Kinase; Tumor immunity; Reproductive science; Developmental science; Rheumatology

NCBI and Uniprot Product Information

• NCBI GI #: 112382270
• NCBI GeneID: 4326
• NCBI Accession #: NP_057239.4
• NCBI GenBank Nucleotide #: NM_016155.4
• Molecular Weight: 57,938 Da
• NCBI Official Full Name: matrix metalloproteinase-17 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 17 (membrane-inserted)
• NCBI Official Symbol: MMP17
• NCBI Official Synonym Symbols: MT4-MMP
• NCBI Protein Information: matrix metalloproteinase-17
• UniProt Protein Name: Matrix metalloproteinase-17
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP17
• UniProt Synonym Gene Names: MT4MMP; MMP-17; MT-MMP 4; MTMMP4; MT4-MMP; MT4MMP
• UniProt Entry Name: MMP17_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene is considered a member of the membrane-type MMP (MT-MMP) subfamily. However, this protein is unique among the MT-MMP's in that it is a GPI-anchored protein rather than a transmembrane protein. The protein activates MMP-2 by cleavage. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP17: Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Activator; Membrane protein, GPI anchor; Membrane protein, integral; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: proteinaceous extracellular matrix; integral to plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; proteolysis