IL-33 protein

IL-33

Cat. Num. AAA8532863

• Gene Names: Il33; RGD1311155
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: IL-33; Interleukin-33; Interleukin-1 Family Member 11; IL-1F11; Nuclear Factor From High Endothelial Venules; NF-HEV; IL33; C9orf26; IL1F11; NFHEV; IL-33 protein

• Host: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized
• Sequence Length: 264

• Expression System: E Coli
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.; It is not recommended to reconstitute to a concentration less than 100 mug/ml.; Dissolve the lyophilized protein in ddH2O.; Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
• Preparation and Storage: Lyophilized protein should be stored at <-20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at <-20 degree C for 3 months.

Related Product Information for IL-33 protein

Background: Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.
Description: Recombinant Human Interleukin-33 is produced by our E Coli expression system and the target gene encoding Ser112-Thr270 is expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 62079057
• NCBI GeneID: 361749
• NCBI Accession #: NP_001014188.1
• NCBI GenBank Nucleotide #: NM_001014166.1
• UniProt Accession #: Q66H70
• Molecular Weight: 29,486 Da
• NCBI Official Full Name: interleukin-33
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: Il33
• NCBI Official Synonym Symbols: RGD1311155
• NCBI Protein Information: interleukin-33
• UniProt Protein Name: Interleukin-33
• UniProt Gene Name: Il33
• UniProt Synonym Gene Names: IL-33

Uniprot Description

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury ().