Soluble Fibrin Active Protein | FGA active protein

Soluble Human Fibrin

Cat. Num. AAA136120

• Gene Names: FGA; Fib2
• Purity: >95% by SDS-PAGE analysis
• Synonyms: Soluble Fibrin; Soluble Human Fibrin; FGA active protein

• Host: Human plasma
• Purity/Purification: >95% by SDS-PAGE analysis
• Form/Format: Frozen liquid; 0.02M Acetic Acid; pH 3.6
• Concentration: 4.0mg/ml (varies by lot)
• Sequence Length: 644

• Extintion Coefficient: 1.51
• Specific Activity: >80% Clottable
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -70 degree C; Expiration: 3 years from delivery; Shipping: Dry Ice

Related Product Information for FGA active protein

Normal human plasma clots are dissociated using non-denaturing conditions in which non fibrin bound plasma proteins are removed. The soluble human fibrin clot is then stored under acidic conditions. The soluble human fibrin will reassemble into an insoluble clot when pH is restored to neutral (e.g. by adding one-tenth volume of 10X PBS to the soluble fibrin). SDS-PAGE shows partially crosslinked fibrin with no observable free alpha, beta or gamma chain subunits. No detectable thrombin or plasmin activity is observed in the final product.

Product Categories/Family for FGA active protein

Proteins; Fibrinogen; Human Fibrinogen

NCBI and Uniprot Product Information

• NCBI GI #: 11761629
• NCBI GeneID: 2243
• NCBI Accession #: NP_068657.1
• NCBI GenBank Nucleotide #: NP_068657.1
• UniProt Accession #: P02671; P02675; P02679
• Molecular Weight: 340000
• NCBI Official Full Name: fibrinogen alpha chain isoform alpha
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: FGA
• NCBI Official Synonym Symbols: Fib2
• NCBI Protein Information: fibrinogen alpha chain
• UniProt Protein Name: Fibrinogen alpha chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGA
• UniProt Entry Name: FIBA_HUMAN

NCBI Description

This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. [provided by RefSeq, Jan 2016]

Uniprot Description

FGA: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGA are a cause of congenital afibrinogenemia (CAFBN). This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha- dysfibrinogenemias. Defects in FGA are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: extracellular space; cell surface; fibrinogen complex; plasma membrane; extracellular region; cell cortex; vesicle; external side of plasma membrane

Molecular Function: protein binding, bridging; protein binding; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; platelet activation; extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; cell-matrix adhesion; signal transduction; platelet degranulation; cellular protein complex assembly; positive regulation of protein secretion; positive regulation of vasoconstriction; innate immune response; blood coagulation; response to calcium ion; positive regulation of exocytosis

Disease: Amyloidosis, Familial Visceral; Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital