BACE, Asp2 Beta-Secretase Peptide Substrate Peptide | BACE1 peptide
BACE, Asp2 Beta-Secretase Peptide Substrate, Human (Memapsin2, Beta-site APP Cleaving Enzyme, Aspartyl Protease 2)
Highly purified
Highly purified
Recently, BACE (Beta-site APP Cleaving Enzyme) has been identified as b-secretase. BACE belongs to the family of Aspartyl proteases (Asp) also known as Memapsins. At least four related Asps, located on chromosome IV and X, have been cloned (Asp1, Asp2, Asp3, and Asp4). Human BACE/Asp2/Memapsin2, located on chromosome 11, is a transmembrane protein of 501 aa (signal peptide 1-21 aa, a proprotein domain 22-45 aa, 1 TM domain near the CT, and a short cytoplasmic CT- tail of 24 aa; mature protein 46-460 aa). The lumenal portion of BACE has two active site motifs at 93 aa and 289 aa with signature sequence of aspartic proteases. Rat and mouse BACE (501 aa) are 96% identical with human BACE. BACE expression was most prominent in most areas of the rat brain and pancreas. It has been localized in the compartments of the secretory pathways.
NCBI and Uniprot Product Information
NCBI Description
Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]
Uniprot Description
Function: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Ref.15 Ref.24
Catalytic activity: Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer amyloid precursor protein.
Enzyme regulation: Inhibited by RTN3 and RTN4. Ref.20 Ref.21
Subunit structure: Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4. Interacts with SNX6. Interacts with PCSK9. Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24
Subcellular location: Membrane; Single-pass type I membrane protein. Golgi apparatus › trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane. Note: Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. Ref.17 Ref.24
Tissue specificity: Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. Ref.15 Ref.16
Domain: The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. Ref.17
Post-translational modification: Glycosylated. Ref.16
Sequence similarities: Belongs to the peptidase A1 family.
Sequence caution: The sequence BAA86463.2 differs from that shown. Reason: Frameshift at position 34.
Research Articles on BACE1
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Product Notes
The BACE1 bace1 (Catalog #AAA659240) is a Peptide produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BACE, Asp2 Beta-Secretase Peptide Substrate can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Researchers should empirically determine the suitability of the BACE1 bace1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACE, Asp2 Beta-Secretase Peptide Substrate, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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