Cathepsin G Native Protein | CTSG native protein

Human Cathepsin G

Cat. Num. AAA318181

• Gene Names: CTSG; CG; CATG; MGC23078
• Purity: >95% pure (SDS-PAGE)
• Synonyms: Cathepsin G; Human Cathepsin G; CTSG native protein

• Host: Human Neutrophil
• Purity/Purification: >95% pure (SDS-PAGE)
• Form/Format: Purified; We suggest using 50mM Na Acetate, pH 5.5, with 150mM Sodium Chloride as the buffer to be added to the salt-free lyophilized solid.
• Sequence Length: 255

• Application Notes: Specific methodologies have not been tested using this product.
• Inactivation: Not applicable
• Buffer: Not applicable
• Preservative: None
• Lyophilized: Yes
• Warnings: All human source materials have tested negative for HIV 1, HIV 2 and non-reactive for anti-HCV, HBsAg and anti-HBc. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be conside
• Concentration Note: Not applicable; Activity: 2 to 4 Units/mg protein. One unit of activity is defined as the amount of enzyme that hydrolyzes one micromole of Suc-ala-ala-pro-phe-pNA (1mM) per minute at 25 degree C in 160mM Tris-HCl, pH 7.4 containing 1.6M Sodium chloride
• Preparation and Storage: Store at -20 degree C. Avoid multiple freeze/thaw cycles.

Product Categories/Family for CTSG native protein

Purified Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 179915
• NCBI GeneID: 1511
• UniProt Accession #: P08311; Q6IBJ6; Q9UCA5; Q9UCU6
• NCBI Official Full Name: cathepsin G
• NCBI Official Synonym Full Names: cathepsin G
• NCBI Official Symbol: CTSG
• NCBI Official Synonym Symbols: CG; CATG; MGC23078
• NCBI Protein Information: cathepsin G; OTTHUMP00000178752
• UniProt Protein Name: Cathepsin G
• Protein Family: Cathepsin
• UniProt Gene Name: CTSG
• UniProt Entry Name: CATG_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq]

Uniprot Description

Function: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. Ref.6 Ref.9 Ref.11

Catalytic activity: Specificity similar to chymotrypsin C.

Enzyme regulation: Inhibited by soybean trypsin inhibitor, benzamidine, the synthetic peptide R13K, Z-Gly-Leu-Phe-CH2Cl, phenylmethylsulfonyl fluoride, 3,4-dichloroisocoumarin, DFP, SBTI and alpha-1-antitrypsin. Inhibited by LPS from P.aeruginosa but not by LPS from S.minnesota. Not inhibited by elastinal, CMK, TLCK and ETDA. Ref.6 Ref.9 Ref.11

Subcellular location: Cell surface Ref.6.

Sequence similarities: Belongs to the peptidase S1 family.Contains 1 peptidase S1 domain.

Biophysicochemical propertiesKinetic parameters:KM=1.15 mM for Z-Lys-SBzl Ref.6KM=0.26 mM for Suc-Ala-Ala-Pro-Phe-SBzl