H2A Histone Family, Member Z (H2AFZ) ELISA Kit | H2AFZ elisa kit

Multi-species H2A Histone Family, Member Z (H2AFZ) ELISA Kit

Cat. Num. AAA2706750

• Gene Names: H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
• Reactivity: Human, Mouse, Rat
• Synonyms: H2A Histone Family; Member Z (H2AFZ); Multi-species H2A Histone Family; Member Z (H2AFZ) ELISA Kit; H2A.z; H2A/z; H2AZ; H2AFZ elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of H2A Histone Family, Member Z (H2AFZ).; No significant cross-reactivity or interference between H2A Histone Family, Member Z (H2AFZ) and analogues was observed.
• Sequence Length: 128

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H2A Histone Family, Member Z (H2AFZ) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H2A Histone Family, Member Z (H2AFZ) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for H2AFZ elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2A Histone Family, Member Z (H2AFZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2A Histone Family, Member Z (H2AFZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2A Histone Family, Member Z (H2AFZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H2A Histone Family, Member Z (H2AFZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 74355514
• NCBI GeneID: 3015
• Molecular Weight: 13,553 Da
• NCBI Official Full Name: H2A histone family, member Z
• NCBI Official Synonym Full Names: H2A histone family member Z
• NCBI Official Symbol: H2AFZ
• NCBI Official Synonym Symbols: H2AZ; H2A.z; H2A/z; H2A.Z-1
• NCBI Protein Information: histone H2A.Z
• UniProt Protein Name: Histone H2A.Z
• UniProt Gene Name: H2AFZ
• UniProt Synonym Gene Names: H2AZ; H2A/z

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Uniprot Description

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.