GLUT1 elisa kit

GLUT1 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500258

• Gene Names: SLC2A1; CSE; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; SDCHCN; GLUT1DS
• Reactivity: Human, Mouse, Rat
• Synonyms: GLUT1; GLUT1 Colorimetric Cell-Based ELISA Kit; Solute carrier family 2 facilitated glucose transporter member 1; Glucose transporter type 1 erythrocyte/brain; GLUT-1; HepG2 glucose transporter; SLC2A1; GLUT1 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 492

• Preparation and Storage: Store at 4 degree C.

Related Product Information for GLUT1 elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target. The eIF2alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor eIF2alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of eIF2alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eIF2alpha.

Product Categories/Family for GLUT1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 166795299
• NCBI GeneID: 6513
• NCBI Accession #: NP_006507.2
• NCBI GenBank Nucleotide #: NM_006516.2
• UniProt Accession #: P11166; O75535; Q147X2; A8K9S6; B2R620; D3DPX0
• Molecular Weight: 54084
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 1
• NCBI Official Synonym Full Names: solute carrier family 2 member 1
• NCBI Official Symbol: SLC2A1
• NCBI Official Synonym Symbols: CSE; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; SDCHCN; GLUT1DS
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 1
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 1
• Protein Family: Glucose transporter
• UniProt Gene Name: SLC2A1
• UniProt Synonym Gene Names: GLUT1; GLUT-1

NCBI Description

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

Uniprot Description

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; cortical actin cytoskeleton; cytosol; female pronucleus; Golgi membrane; integral component of plasma membrane; melanosome; membrane; midbody; plasma membrane; sarcolemma; Z disc

Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transmembrane transporter activity; glucose transmembrane transporter activity; identical protein binding; kinase binding; protein binding; protein self-association; xenobiotic transporter activity

Biological Process: cellular response to glucose starvation; cerebral cortex development; female pregnancy; glucose transport; L-ascorbic acid metabolic process; lactose biosynthetic process; protein complex assembly; regulation of insulin secretion; response to insulin stimulus; xenobiotic transport

Disease: Stomatin-deficient Cryohydrocytosis With Neurologic Defects