VASP Antibody | anti-VASP antibody

VASP (Ab-157) Antibody

Cat. Num. AAA856845

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: VASP; VASP (Ab-157) Antibody; vasodilator-stimulated phosphoprotein; anti-VASP antibody

• Reactivity: Human, Mouse, Rat
• Specificity: VASP (Ab-157) antibody detects endogenous levels of total VASP protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 380

• Applicable Applications for anti-VASP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells untreated or treated with forskolin (40muM, 30min), using VASP (Ab-157) antibody and VASP (phospho-Ser157) antibody.)

Product Categories/Family for anti-VASP antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Zhao WM, et al. (2001) EMBO J 20(9): 2315-2325. Millard TH, et al. (2005) EMBO J 24(2): 240-250. Kühnel K, et al. (2004) Proc Natl Acad Sci U S A 101(49): 17027-17032. Haffner C, et al. (1995) EMBO J 14(1): 19-27. Ramesh N, et al. (1997) Proc Natl Acad Sci U S A 94(26): 14671-14676.

NCBI and Uniprot Product Information

• NCBI GI #: 4507869
• NCBI GeneID: 7408
• NCBI Accession #: NP_003361.1
• NCBI GenBank Nucleotide #: NM_003370.3
• UniProt Accession #: P50552; Q6PIZ1; Q93035; B2RBT9
• Molecular Weight: 39,830 Da
• NCBI Official Full Name: vasodilator-stimulated phosphoprotein
• NCBI Official Synonym Full Names: vasodilator stimulated phosphoprotein
• NCBI Official Symbol: VASP
• NCBI Protein Information: vasodilator-stimulated phosphoprotein
• UniProt Protein Name: Vasodilator-stimulated phosphoprotein
• Protein Family: Vasodilator-stimulated phosphoprotein
• UniProt Gene Name: VASP
• UniProt Synonym Gene Names: VASP

NCBI Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Uniprot Description

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.