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Testing Data (P-peptide-+)

TNNI3 Antibody | anti-TNNI3 antibody

TNNI3 (Phospho-Ser22+Ser23) Antibody

Gene Names
TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
TNNI3; TNNI3 (Phospho-Ser22+Ser23) Antibody; TNNC1; troponin I; cardiac muscle; anti-TNNI3 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
TNNI3 (Phospho-Ser22+Ser23) antibody detects endogenous levels of TNNI3 only when phosphorylated at serine 22.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
210
Applicable Applications for anti-TNNI3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human TNNI3 around the phosphorylation site of serine 22 (R-R-SP-SP-A-N).
All Sites
Human: Ser22+Ser23
Mouse: Ser22+Ser23
Rat: Ser22+Ser23
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using TNNI3 (Phospho-Ser22+Ser23) antibody. Western blot analysis of extracts from mouse heart cells, using TNNI3 (Phospho-Ser22+Ser23) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using TNNI3 (Phospho-Ser22+Ser23) antibody. Western blot analysis of extracts from mouse heart cells, using TNNI3 (Phospho-Ser22+Ser23) antibody.)
References
Raffaella Di Lisi, J. Biol. Chem., Sep 1998; 273: 25371. Emile Missov, Circulation, Nov 1997; 96: 2953-2958. Brandon J. Biesiadecki, J. Biol. Chem., Apr 2004; 279: 13825-13832.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,008 Da
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I3, cardiac type
NCBI Official Symbol
TNNI3
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
Protein Family
UniProt Gene Name
TNNI3
UniProt Synonym Gene Names
TNNC1

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Uniprot Description

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Research Articles on TNNI3

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Product Notes

The TNNI3 tnni3 (Catalog #AAA858949) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The TNNI3 (Phospho-Ser22+Ser23) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNNI3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the TNNI3 tnni3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNNI3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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