Western Blot (WB)
(Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat (3), Hela(4) and HEK293 (5) cell lysate.)
Mouse anti-Human ERN1 Antibody | anti-ERN1 antibody
ERN1 Antibody
IHC: 1:200-1:1000
ELISA: 1:10000
Western Blot (WB)
(Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat (3), Hela(4) and HEK293 (5) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat (3), Hela(4) and HEK293 (5) cell lysate.)
Immunohistochemistry (IHC)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ERN1 mouse mAb)
Immunohistochemistry (IHC)
(Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ERN1 mouse mAb)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
Uniprot Description
Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875).
Research Articles on ERN1
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Product Notes
The ERN1 ern1 (Catalog #AAA850823) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ERN1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the ERN1 ern1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERN1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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