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Western Blot (WB) (Western blot analysis of extracts from 293 cells, using DNA Polymerase lambda antibody.)

DNA Polymerase lambda Antibody | anti-POLL antibody

DNA Polymerase lambda Antibody

Gene Names
POLL; BETAN; POLKAPPA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DNA Polymerase lambda; DNA Polymerase lambda Antibody; DNA polymerase lambda; EC 2.7.7.7; EC 4.2.99.-; Pol Lambda; DNA polymerase kappa; DNA polymerase beta-2; Pol beta2; POLL; anti-POLL antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
DNA Polymerase lambda antibody detects endogenous levels of total DNA Polymerase lambda protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
575
Applicable Applications for anti-POLL antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human DNA Polymerase lambda.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, using DNA Polymerase lambda antibody.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells, using DNA Polymerase lambda antibody.)
References
Aoufouchi S., Nucleic Acids Res. 28:3684-3693(2000). Nagasawa K.I., J. Biol. Chem. 275:31233-31238(2000). Ota T., Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,839 Da
NCBI Official Full Name
DNA polymerase lambda isoform a
NCBI Official Synonym Full Names
DNA polymerase lambda
NCBI Official Symbol
POLL
NCBI Official Synonym Symbols
BETAN; POLKAPPA
NCBI Protein Information
DNA polymerase lambda
UniProt Protein Name
DNA polymerase lambda
Protein Family
UniProt Gene Name
POLL
UniProt Synonym Gene Names
Pol beta2

NCBI Description

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

DNA polymerase that functions in several pathways of DNA repair (PubMed:11457865, PubMed:19806195, PubMed:20693240). Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PubMed:11457865, PubMed:19806195). Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination (PubMed:19806195, PubMed:20693240). Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities (PubMed:10982892, PubMed:10887191, PubMed:12809503, PubMed:14627824, PubMed:15537631, PubMed:19806195). Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (PubMed:11457865, PubMed:19806195).

Research Articles on POLL

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Product Notes

The POLL poll (Catalog #AAA858271) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The DNA Polymerase lambda Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Polymerase lambda can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:5000. Researchers should empirically determine the suitability of the POLL poll for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Polymerase lambda, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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