DDX5/DEAD-box Protein 5 Antibody | anti-DDX5 antibody

DDX5/DEAD-box Protein 5 Antibody

Cat. Num. AAA8509642

• Gene Names: DDX5; p68; HLR1; G17P1; HUMP68
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: DDX5/DEAD-box Protein 5; DDX5/DEAD-box Protein 5 Antibody; DEAD-box protein 5; DEAD-box protein p68; EC 3.6.1.-; HELR; HLR1; Probable RNA-dependent helicase p68; anti-DDX5 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: DDX5/DEAD-box Protein 5 antibody detects endogenous levels of total DDX5/DEAD-box Protein 5 protein.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 614

• Applicable Applications for anti-DDX5 antibody: Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from Internal of human DDX5/DEAD-box Protein 5.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HeLa cells, using DDX5/DEAD-box Protein 5 antibody.)

References

Hloch P., Nucleic Acids Res. 18:3045-3045(1990). Roessler O.G., Nucleic Acids Res. 28:932-939(2000). Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 1002341719
• NCBI GeneID: 1655
• NCBI Accession #: NP_001307524.1
• NCBI GenBank Nucleotide #: NM_001320595.1
• UniProt Accession #: P17844; O75681; Q53Y61; B4DLW8; B5BU21; D3DU32; E7ETL9
• Molecular Weight: 60,563 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX5 isoform a
• NCBI Official Synonym Full Names: DEAD-box helicase 5
• NCBI Official Symbol: DDX5
• NCBI Official Synonym Symbols: p68; HLR1; G17P1; HUMP68
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX5
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX5
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX5
• UniProt Synonym Gene Names: G17P1; HELR; HLR1

NCBI Description

This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]

Uniprot Description

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.