Mouse interleukin 4 ELISA Kit | IL4 elisa kit

Mouse Interleukin 4, IL-4 ELISA Kit

Cat. Num. AAA704414

• Gene Names: Il4; Il-4; BSF-1
• Reactivity: Mouse
• Synonyms: interleukin 4; Mouse Interleukin 4; IL-4 ELISA Kit; IL-4 ELISA KIT; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; IL4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of chicken IL-4. No significant cross-reactivity or interference between chicken IL-4 and analogues was observed.
• Sequence Length: 140

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/mL-2000 pg/mL
• Sensitivity: 7.81 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 10946584
• NCBI GeneID: 16189
• NCBI Accession #: NP_067258.1
• NCBI GenBank Nucleotide #: NM_021283.2
• UniProt Accession #: P07750
• Molecular Weight: 15,834 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: Il4
• NCBI Official Synonym Symbols: Il-4; BSF-1
• NCBI Protein Information: interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: Il4
• UniProt Synonym Gene Names: Il-4; IL-4; BSF-1
• UniProt Entry Name: IL4_MOUSE

Uniprot Description

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.