VE-Cadherin Phospho-Regulation Antibody Sampler Kit
VE-Cadherin Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Synonyms
VE-Cadherin Phospho-Regulation; Cadherin VEK; VE-Cadherin Phospho-Regulation antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for VE-Cadherin Phospho-Regulation antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for VE-Cadherin Phospho-Regulation antibody sampler kit
Kit Summary: The VE-cadherin phospho-regulation antibody sampler kit can be used to detect the phosphorylation of Tyr-685 relative tototal expression of VE-cadherin. The kit includes a phospho-specific antibody to detect VE-cadherin Tyr-685, and mouse monoclonal and rabbit polyclonal antibodies to examine total VE-cadherin expression. The kit also includes secondary reagents for detection of primary antibodies in various applications.
Background: Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation.Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediatedadhesion may be by the juxta membrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found inendothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmicdomain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminaldomain that interacts with beta- or gamma-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosinesites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation ofTyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.
Background: Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation.Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediatedadhesion may be by the juxta membrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found inendothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmicdomain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminaldomain that interacts with beta- or gamma-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosinesites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation ofTyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.
Similar Products
Product Notes
The Human, Rat, Mouse VE-Cadherin Phospho-Regulation (Catalog #AAA474212) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474212 Antibody Sampler Kit recognizes Human, Rat, Mouse VE-Cadherin Phospho-Regulation. AAA Biotech's VE-Cadherin Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the VE-Cadherin Phospho-Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VE-Cadherin Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
