PDK1 Phospho-Regulation Antibody Sampler Kit | PDK1 antibody sampler kit
PDK1 Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Synonyms
PDK1 Phospho-Regulation; PDK1 antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for PDK1 antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for PDK1 antibody sampler kit
Kit Summary: The PDK1 phospho-regulation antibody sampler kit can be used to examine phosphorylation of PDK1 at Ser-241 and Tyr-9. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of PDK1, as well assecondary reagents for antibody detection.
Background: 3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survivalkinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs.PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstreamkinases like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cellsurvival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid secondmessengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation isrequired for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylationmay also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 isimportant for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.
Background: 3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survivalkinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs.PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstreamkinases like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cellsurvival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid secondmessengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation isrequired for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylationmay also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 isimportant for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.
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Product Notes
The Human, Rat, Mouse PDK1 (Catalog #AAA474492) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474492 Antibody Sampler Kit recognizes Human, Rat, Mouse PDK1. AAA Biotech's PDK1 Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the PDK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDK1 Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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