p130 Cas Phospho-Regulation Antibody Sampler Kit | p130 antibody sampler kit
p130 Cas Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
p130 Cas Phospho-Regulation; p130 antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for p130 antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for p130 antibody sampler kit
Kit Summary: The p130 Cas phospho-regulation antibody sampler kit can be used to detect p130 Cas phosphorylation at Tyr-751 andTyr-762. The kit also includes an antibody to examine total p130 Cas expression levels and secondary reagents for rabbitpolyclonal and mouse monoclonal antibody detection.
Background: p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking proteincontaining multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switchregulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Srcbinding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs).Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas centralsubstrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, andTyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTBdomains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site canattenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation.
Background: p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking proteincontaining multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switchregulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Srcbinding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs).Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas centralsubstrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, andTyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTBdomains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site canattenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation.
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Product Notes
The Human, Rat, Mouse p130 (Catalog #AAA474489) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474489 Antibody Sampler Kit recognizes Human, Rat, Mouse p130. AAA Biotech's p130 Cas Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the p130 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p130 Cas Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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