Western Blot (WB)
(Western Blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 or the same antibody preincubated with Phospho-Akt (Thr308) Blocking Peptide.)
Akt Peptide | Akt1 peptide
Akt, phosphorylated (Thr308) Control Peptide
Purified
Purified
Immunohistochemistry: Add 2ul of antibody and 5ul of blocking peptide to 153ul of buffer, and
incubate at least 30 minutes before allowing to react with the slides.
Western Blot (WB)
(Western Blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 or the same antibody preincubated with Phospho-Akt (Thr308) Blocking Peptide.)
Western Blot (WB)
(Western Blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 or the same antibody preincubated with Phospho-Akt (Thr308) Blocking Peptide.)
Western Blot (WB)
(Western Blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-Akt (Thr308) Antibody #9275 or the same antibody preincubated with Phospho-Akt (Thr308) Blocking Peptide.)
Western Blot (WB)
(Western Blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-Akt (Thr308) Antibody #9275 or the same antibody preincubated with Phospho-Akt (Thr308) Blocking Peptide.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Akt (Thr308) (244F1) Rabbit mAb #4056 preincubated with an unrelated control peptide (left) or with Phospho-Akt (Thr308) Blocking Peptide (right).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Akt (Thr308) (244F1) Rabbit mAb #4056 preincubated with an unrelated control peptide (left) or with Phospho-Akt (Thr308) Blocking Peptide (right).)
NCBI and Uniprot Product Information
Uniprot Description
Function: Serine/threonine kinase involved in various developmental processes. During early embryogenesis, acts as a survival protein. During mid-embryogenesis, phosphorylates and activates trh, a transcription factor required for tracheal cell fate determination. Also regulates tracheal cell migration. Later in development, acts downstream of PI3K and Pk61C/PDK1 in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and antagonizing FOXO transcription factor. Controls follicle cell size during oogenesis. May also stimulate cell growth by phosphorylating Gig/Tsc2 and inactivating the Tsc complex. Dephosphorylation of 'Ser-586' by Phlpp triggers apoptosis and suppression of tumor growth. Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17
Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.2
Subcellular location: Cytoplasm › cytosol. Cell membrane. Note: Recruited to plasma membrane upon activation.
Tissue specificity: Ubiquitously expressed. Present in ovary, where it is concentrated at the basal side of follicle cells. Ref.2
Developmental stage: Expressed both maternally and zygotically. Strongly expressed in embryo and pupa. Weakly expressed in larva. Mildly expressed in adult. Ref.2 Ref.17
Domain: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in its targeting to the plasma membrane
Probable.
Post-translational modification: Phosphorylated and activated by Pk61C/PDK1. Phosphorylated on Ser-586 by the rictor-Tor complex. Ref.8 Ref.10 Ref.18 Ref.19
Disruption phenotype: Death at the first instar larval stage. Ref.6
Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 protein kinase domain.
Research Articles on Akt1
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Product Notes
The Akt1 akt1 (Catalog #AAA659552) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. The Akt, phosphorylated (Thr308) Control Peptide reacts with Human, Mouse and may cross-react with other species as described in the data sheet. Applications: Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western immunoblotting and immunohistochemistry protocols. Immunohistochemistry: Add 2ul of antibody and 5ul of blocking peptide to 153ul of buffer, and incubate at least 30 minutes before allowing to react with the slides. Researchers should empirically determine the suitability of the Akt1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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