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Testing Data (Peptide-+)

anti-Human, Mouse SENP5 Antibody | anti-SENP5 antibody

SENP5 Antibody

Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
SENP5; SENP5 Antibody; EC 3.4.22.-; SUMO1/sentrin specific peptidase 5; anti-SENP5 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
SENP5 antibody detects endogenous levels of total SENP5 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
709
Applicable Applications for anti-SENP5 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human SENP5.
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using SENP5 antibody. Western blot analysis of extracts from COS7 cells, using SENP5 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using SENP5 antibody. Western blot analysis of extracts from COS7 cells, using SENP5 antibody.)
References
Alessandra Di Bacco, Mol. Cell. Biol., Jun 2006; 26: 4489-4498. Thomas Colby, Plant Physiology, Sep 2006; 142: 318-332. Pei-Zheng Zheng, PNAS, May 2005; 102: 7653-7658. Rodolfo Zunino, J. Cell Sci., Apr 2007; 120: 1178-1188.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,310 Da
NCBI Official Full Name
sentrin-specific protease 5 isoform 2
NCBI Official Synonym Full Names
SUMO specific peptidase 5
NCBI Official Symbol
SENP5
NCBI Protein Information
sentrin-specific protease 5
UniProt Protein Name
Sentrin-specific protease 5
Protein Family
UniProt Gene Name
SENP5

NCBI Description

The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP5, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM, Jun 2009]

Uniprot Description

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.

Research Articles on SENP5

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Product Notes

The SENP5 senp5 (Catalog #AAA854515) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The SENP5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SENP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the SENP5 senp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SENP5, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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