CEACAM5 recombinant protein

CEACAM5 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120676

• Gene Names: CEACAM5; CEA; CD66e
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CEACAM5; CEACAM5 Protein; Human; Recombinant (hFc Tag); Human CEACAM5/CD66e Protein (Fc Tag); CD66e Protein; CEA Protein; carcinoembryonic antigen-related cell adhesion molecule 5; CEACAM5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala685

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Lys 35
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CEACAM5 (P06731.3)(Met1-Ala685) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CEACAM5 recombinant protein

Background: CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of the immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with an MW of 180kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domains and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.

Product Categories/Family for CEACAM5 recombinant protein

Cancer Drug Targets

References

Baczyska D, et al. (2003) The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression. Cell Mol Biol Lett. 8(2): 471-86.
Blumenthal RD, et al. (2005) Inhibition of adhesion, invasion, and metastasis by antibodies targeting CEACAM6 (NCA-90) and CEACAM5 (Carcinoembryonic Antigen). Cancer Res. 65(19): 8809-17.
Liebig B, et al. (2005) Forced expression of deltaN-TCF-1B in colon cancer derived cell lines is accompanied by the induction of CEACAM5/6 and mesothelin. Cancer Lett. 223(1): 159-67.

NCBI and Uniprot Product Information

• NCBI GI #: 317373456
• NCBI GeneID: 1048
• UniProt Accession #: P06731; H9KVA7
• Molecular Weight: 76,795 Da
• NCBI Official Full Name: Carcinoembryonic antigen-related cell adhesion molecule 5
• NCBI Official Synonym Full Names: carcinoembryonic antigen-related cell adhesion molecule 5
• NCBI Official Symbol: CEACAM5
• NCBI Official Synonym Symbols: CEA; CD66e
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 5
• Protein Family: Carcinoembryonic antigen-related cell adhesion molecule
• UniProt Gene Name: CEACAM5
• UniProt Synonym Gene Names: CEA; CEA
• UniProt Entry Name: CEAM5_HUMAN

NCBI Description

Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]

Uniprot Description

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis