anti-Human, Mouse RAD52 Antibody | anti-RAD52 antibody

RAD52 (Phospho-Tyr104) Antibody

Cat. Num. AAA859236

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: RAD52; RAD52 (Phospho-Tyr104) Antibody; DNA repair protein RAD52 homolog; RA52; anti-RAD52 antibody

• Reactivity: Human, Mouse
• Specificity: RAD52 (Phospho-Tyr104) antibody detects endogenous levels of RAD52 only when phosphorylated at tyrosine 104.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 418

• Applicable Applications for anti-RAD52 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human RAD52 around the phosphorylation site of tyrosine 104 (K-F-YP-V-G).
• All Sites: Human: Tyr104; Mouse: Tyr105
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from COS cells, treated with H2O2 (100uM, 30mins), using RAD52 (Phospho-Tyr104) antibody.)

Product Categories/Family for anti-RAD52 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Shen Z., Genomics 25:199-206(1995). Muris D.F., Mutat. Res. 315:295-305(1994). Matsuoka S., Science 316:1160-1166(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 661903001
• NCBI GeneID: 5893
• NCBI Accession #: NP_001284348.1
• NCBI GenBank Nucleotide #: NM_001297419.1
• UniProt Accession #: P43351; Q13205; Q9Y5T7; Q9Y5T8; Q9Y5T9
• Molecular Weight: 12,943 Da
• NCBI Official Full Name: DNA repair protein RAD52 homolog isoform a
• NCBI Official Synonym Full Names: RAD52 homolog, DNA repair protein
• NCBI Official Symbol: RAD52
• NCBI Protein Information: DNA repair protein RAD52 homolog
• UniProt Protein Name: DNA repair protein RAD52 homolog
• Protein Family: RAD52 motif-containing protein
• UniProt Gene Name: RAD52

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Uniprot Description

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.