Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of extracts from Jurkat cells and 293 cells, treated with etoposide (25uM, 1hour), using PPGB (32k, Cleaved-Arg326) antibody.)

anti-Human, Mouse PPGB Antibody | anti-PPGB antibody

PPGB (32k, Cleaved-Arg326) Antibody

Gene Names
CTSA; GSL; GLB2; NGBE; PPCA; PPGB
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
PPGB; PPGB (32k; Cleaved-Arg326) Antibody; Lysosomal protective protein [Precursor]; EC 3.4.16.5; Cathepsin A; Carboxypeptidase C; Protective protein for beta-galactosidase; Lysosomal protective protein 32 kDa chain; Lysosomal protective protein 20 kDa chain; CTSA; anti-PPGB antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
PPGB (32k, Cleaved-Arg326) antibody detects endogenous levels of fragment of activated PPGB resulting from cleavage adjacent to Arg326.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
498
Applicable Applications for anti-PPGB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from human PPGB.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells and 293 cells, treated with etoposide (25uM, 1hour), using PPGB (32k, Cleaved-Arg326) antibody.)

Western Blot (WB) (Western blot analysis of extracts from Jurkat cells and 293 cells, treated with etoposide (25uM, 1hour), using PPGB (32k, Cleaved-Arg326) antibody.)
References
Galjart N.J., Cell 54:755-764(1988). Deloukas P., Nature 414:865-871(2001). Jackman H.L., J. Biol. Chem. 265:11265-11272(1990).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,489 Da
NCBI Official Full Name
lysosomal protective protein isoform a
NCBI Official Synonym Full Names
cathepsin A
NCBI Official Symbol
CTSA
NCBI Official Synonym Symbols
GSL; GLB2; NGBE; PPCA; PPGB
NCBI Protein Information
lysosomal protective protein
UniProt Protein Name
Lysosomal protective protein
UniProt Gene Name
CTSA
UniProt Synonym Gene Names
PPGB; PPCA

NCBI Description

This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]

Uniprot Description

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Research Articles on PPGB

Similar Products

Product Notes

The PPGB ctsa (Catalog #AAA851667) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PPGB (32k, Cleaved-Arg326) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPGB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the PPGB ctsa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPGB, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.