Human Lysosomal protective protein ELISA Kit | CTSA elisa kit

Human Lysosomal protective protein ELISA Kit

Cat. Num. AAA764996

• Gene Names: CTSA; GSL; GLB2; NGBE; PPCA; PPGB
• Reactivity: Human
• Synonyms: Lysosomal protective protein; Human Lysosomal protective protein ELISA Kit; CTSA (Lysosomal protective protein)/GLB2/PPCA/GSL/NGBE/PPGB/Lysosomal Carboxypeptidase A/beta-galactosidase 2/beta-galactosidase protective protein/Carboxypeptidase C/Carboxypeptidase L/cathepsin A/protective protein for beta-galactosidase (galactosialidosis)/Protective protein cathepsin A/Protective protein for beta-galactosidase; CTSA elisa kit

• Reactivity: Human
• Sequence Length: 480

• Assay Type: Sandwich
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CTSA elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 189163485
• NCBI GeneID: 5476
• NCBI Accession #: NP_001121167.1
• NCBI GenBank Nucleotide #: NM_001127695.2
• Molecular Weight: 52,489 Da
• NCBI Official Full Name: lysosomal protective protein isoform b preproprotein
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: CTSA
• NCBI Official Synonym Symbols: GSL; GLB2; NGBE; PPCA; PPGB
• NCBI Protein Information: lysosomal protective protein
• UniProt Protein Name: Lysosomal protective protein
• Protein Family: Lysosomal protective protein
• UniProt Gene Name: CTSA
• UniProt Synonym Gene Names: PPGB; PPCA
• UniProt Entry Name: PPGB_HUMAN

NCBI Description

This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]

Uniprot Description

CTSA: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. Defects in CTSA are the cause of galactosialidosis (GSL). A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. Belongs to the peptidase S10 family.

Protein type: EC 3.4.16.5; Endoplasmic reticulum; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; lysosomal lumen; lysosome; membrane; nucleoplasm

Molecular Function: carboxypeptidase activity; enzyme activator activity; serine carboxypeptidase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; glycosphingolipid metabolic process; intracellular protein transport; positive regulation of catalytic activity; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; proteolysis; proteolysis involved in cellular protein catabolic process; regulation of protein stability; sphingolipid metabolic process

Disease: Galactosialidosis