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Testing Data (Peptide-+)

anti-Human, Mouse ME2 Antibody | anti-ME2 antibody

ME2 Antibody

Gene Names
ME2; ODS1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ME2; ME2 Antibody; EC 1.1.1.38; malate dehydrogenase; malic enzyme 2; Malic enzyme; mitochondrial; MAOM; NAD-dependent malic enzyme; NAD-ME; pyruvic-malic carboxylase; anti-ME2 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
ME2 antibody detects endogenous levels of total ME2 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
479
Applicable Applications for anti-ME2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human ME2.
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ME2 antibody. Western blot analysis of extracts from LOVO cells and RAW264.7 cells, using ME2 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ME2 antibody. Western blot analysis of extracts from LOVO cells and RAW264.7 cells, using ME2 antibody.)
References
Loeber G., J. Biol. Chem. 266:3016-3021(1991). Yanaihara N., Genomics 72:169-179(2001). Abts H.; Thesis (1995), University of Cologne, Germany.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,586 Da
NCBI Official Full Name
NAD-dependent malic enzyme, mitochondrial isoform 2
NCBI Official Synonym Full Names
malic enzyme 2
NCBI Official Symbol
ME2
NCBI Official Synonym Symbols
ODS1
NCBI Protein Information
NAD-dependent malic enzyme, mitochondrial
UniProt Protein Name
NAD-dependent malic enzyme, mitochondrial
UniProt Gene Name
ME2
UniProt Synonym Gene Names
NAD-ME

NCBI Description

This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

MiscellaneousThis isoenzyme can also use NADP+ but is more effective with NAD+.

Research Articles on ME2

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Product Notes

The ME2 me2 (Catalog #AAA8509882) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ME2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ME2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000. Researchers should empirically determine the suitability of the ME2 me2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ME2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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