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Testing Data (Peptide-+)

anti-Human, Mouse KIF20A Antibody | anti-KIF20A antibody

KIF20A (Ab-528) Antibody

Gene Names
KIF20A; MKLP2; RAB6KIFL
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
KIF20A; KIF20A (Ab-528) Antibody; GG10_2; KI20A; kinesin family 20A; Rab6-interacting kinesin-like; RAB6KIFL; Rabkinesin-6; anti-KIF20A antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
KIF20A (Ab-528) antibody detects endogenous levels of total KIF20A protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
890
Applicable Applications for anti-KIF20A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human KIF20A around the phosphorylation site of serine 528 (E-H-SP-L-Q).
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using KIF20A (Ab-528) antibody. Western blot analysis of extracts from 293 cells, using KIF20A (Ab-528) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using KIF20A (Ab-528) antibody. Western blot analysis of extracts from 293 cells, using KIF20A (Ab-528) antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HuvEc cells, using KIF20A (Ab-528) antibody.)

Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HuvEc cells, using KIF20A (Ab-528) antibody.)
References
Horrevoets A.J.G., Blood 93:3418-3431(1999). Lai F., Gene 248:117-125(2000). The MGC Project Team; Genome Res. 14:2121-2127(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
98,100 Da
NCBI Official Full Name
kinesin-like protein KIF20A
NCBI Official Synonym Full Names
kinesin family member 20A
NCBI Official Symbol
KIF20A
NCBI Official Synonym Symbols
MKLP2; RAB6KIFL
NCBI Protein Information
kinesin-like protein KIF20A
UniProt Protein Name
Kinesin-like protein KIF20A
Protein Family
UniProt Gene Name
KIF20A
UniProt Synonym Gene Names
MKLP2; RAB6KIFL; MKlp2

Uniprot Description

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.

Research Articles on KIF20A

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Product Notes

The KIF20A kif20a (Catalog #AAA8502812) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The KIF20A (Ab-528) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIF20A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the KIF20A kif20a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF20A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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