Human Y+L Amino Acid Transporter 1 (SLC7A7) ELISA Kit | SLC7A7 elisa kit

Human Y+L Amino Acid Transporter 1 (SLC7A7) ELISA Kit

Cat. Num. AAA9714251

• Gene Names: SLC7A7; LPI; LAT3; MOP-2; Y+LAT1; y+LAT-1
• Reactivity: Human
• Synonyms: Y+L Amino Acid Transporter 1 (SLC7A7); Human Y+L Amino Acid Transporter 1 (SLC7A7) ELISA Kit; SLC7A7; LAT3; LPI; MOP-2; Y+LAT1; y+LAT-1; OTTHUMP00000164494; monocyte amino acid permease 2; solute carrier family 7 member 7; y(+)L-type amino acid transporter 1; SLC7A7 elisa kit

• Reactivity: Human
• Specificity: Human Y+L amino acid transporter 1 (SLC7A7) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC7A7. No significant cross-reactivity or interference between Human SLC7A7 and analogues was observed.
• Sequence Length: 511

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Y+L amino acid transporter 1 (SLC7A7) Standard Curve.)

Related Product Information for SLC7A7 elisa kit

Description: This Human Y+L amino acid transporter 1 (SLC7A7) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A7 in samples. An antibody specific for SLC7A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC7A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The SLC7A7 gene encodes the light subunit of a cationic amino acid transporter. Together with the heavy chain, 4F2hc (SLC3A2), the SLC7A7 protein forms a functional heterodimeric transporter on the basolateral cell membrane of epithelial cells that transfers lysine, arginine, and ornithine from the cell to the extracellular space The SLC7A7 gene encodes a deduced 511-residue protein with a molecular mass of 56 kD that contains 12 transmembrane domains. The protein sequence shows 81% homology to the opossum protein. Northern blot analysis identified a 2.4-kb mRNA transcript expressed in kidney, peripheral blood leukocytes, lung, placenta, spleen, and small intestine. SLC7A7 associated with SLC3A2 to form a functional 135-kD heterodimer linked by a disulfide bridge involving cysteine 109 of SLC3A2.

NCBI and Uniprot Product Information

• NCBI GI #: 186910306
• NCBI GeneID: 9056
• NCBI Accession #: NP_001119577.1
• NCBI GenBank Nucleotide #: NP_001119577.1
• UniProt Accession #: Q9UM01
• NCBI Official Full Name: Y+L amino acid transporter 1
• NCBI Official Synonym Full Names: solute carrier family 7 member 7
• NCBI Official Symbol: SLC7A7
• NCBI Official Synonym Symbols: LPI; LAT3; MOP-2; Y+LAT1; y+LAT-1
• NCBI Protein Information: Y+L amino acid transporter 1
• UniProt Protein Name: Y+L amino acid transporter 1
• Protein Family: Y+L amino acid transporter
• UniProt Gene Name: SLC7A7
• UniProt Synonym Gene Names: MOP-2; Y+LAT1; y+LAT-1

NCBI Description

The protein encoded by this gene is the light subunit of a cationic amino acid transporter. This sodium-independent transporter is formed when the light subunit encoded by this gene dimerizes with the heavy subunit transporter protein SLC3A2. This transporter is found in epithelial cell membranes where it transfers cationic and large neutral amino acids from the cell to the extracellular space. Defects in this gene are a cause of lysinuric protein intolerance (LPI). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2011]

Uniprot Description

Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Involved in the transport of L-arginine in monocytes.